Figure 1.

Mcl-1 protein is early and ubiquitously downregulated by UNBS1450 in human cancer cells. Kinetic analysis by western blot of Mcl-1, Bcl-xL and Bcl-2 protein expression in U937 (a) and Jurkat (b) cells following the treatment with UNBS1450 (20 nM). In parallel, caspase activation was assayed by analysis of caspase-3 cleavage and confirmed by caspase-3/7 activity assay (bottom panels). (c) Western blot analysis of the levels of basal expression of Mcl-1, Bcl-2 and Bcl-xL proteins in different human cell lines. (d) Comparative western blot analysis of Mcl-1, Bcl-2 and Bcl-xL expression after UNBS1450 treatment at the corresponding IC50 values for 24 h. One of three independent experiments is shown for the western blot analysis. Values are the mean of three independent experiments +/− S.D. Statistical analysis was performed with one-way ANOVA test (post-hoc test: Sidak). Significance is reported as: **P<0.01, ***P<0.001 respect control values