Figure 2.

TRPV4-induced modulations of apoptosis-related signaling pathways and apoptosis-related proteins. (a–e) Western blot analysis showing the protein levels of p-38 MAPK (a), p-JNK (b), Bcl-2 (c), Bax (d) and the cleaved caspase-3 (e) in the hippocampi of mice injected with vehicle (control mice) and GSK1016790A, respectively. (f and g) TRPV4-induced decrease in Bcl-2/Bax protein ratio (f) and increase in the cleaved caspase-3 protein level (g) were blocked by a PI3K agonist 740 Y-P and a p38 MAPK antagonist SB203580, but were unaffected by a JNK antagonist SP600125. **P<0.01 versus control mice, ^#P<0.05 and ^##P<0.01 versus vehicle-treated GSK1016790A-injected mice