Figure 5.

VDR and ΔNp63α are required for VD₃-mediated cell proliferation. HaCaT and HaCaT II-4 cells were transfected with non-silencing control (NSC) or siVDR (panel a) or sip63 (panel b) followed by treatment with vehicle control, 10 nM or 100 nM VD₃ for 8, 24 and 48 h as indicated. Cell proliferation was measured by MTS cell titer assay. Y axis represents fold change when compared with NSC transfected vehicle-treated cells. Confirmation of silencing was measured by western blot following VD₃ treatment (lower panels). (c) HaCaT and HaCaT II-4 cells were transfected with siRNA against p63 or VDR followed by treatment with vehicle control or VD₃ at 10 nM or 100 nM for 24 and 48 h. Cell viability was measured by trypan blue cell exclusion. Error bars represent standard deviation from the mean. *P values≤0.05 for knockdown condition that is significantly different from vehicle-treated NSC