Figure 5.

Effect of salicylates on HMGB1-induced cell migration is COX-2 independent and is not additive with BoxA. (a and b) SA suppresses HMGB1's chemoattractant activity on mouse 3T3 fibroblasts (a) and MEFs knock out for COX-2 (Ptgs2−/−) (b). Cell migration was performed in Boyden chambers 3 h after stimulation with 1 nM reduced HMGB1, 0.1 μM fMLP or buffer (phosphate-buffered saline (PBS)). Bars represent the mean of triplicate samples (P<0.001 in analysis of variance (ANOVA)). (c) Quantification of wound healing assay of PHI cells in the presence of HMGB1 and ASA or BoxA alone or in combination after 48 h. The percentage of wound reduction was analyzed using the ImageJ software (NIH). Experiments were carried out in triplicate. **P<0.002, HMGB1-treated cells compared with vehicle. ^#P=0.01 and ^##P<0.005, MM cells treated with HMGB1 in the presence of BoxA, ASA or BoxA+ASA compared with cells treated with HMGB1 alone