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. 2015 Jun 18;6(6):e1790. doi: 10.1038/cddis.2015.154

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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IL-1β treatment decreases SERCA2b protein stability in INS-1 cells and isolated rat islets. INS-1 cells (a–c) or isolated rat islets (d–e) were treated with 1 μM actinomycin or 10 μM CHX combined with or without 5 ng/ml of IL-1β for indicated times. Total RNA and protein were isolated, and RNA was subjected to quantitative real-time PCR (qRT-PCR) for quantification of SERCA2b and actin transcript levels. Immunoblot was performed using antibodies against SERCA2 and actin. Protein and mRNA levels were plotted relative to levels at time zero, and one-phase decay lines for each treatment are shown. Indicated comparisons are significantly different (*P<0.05 and ***P<0.001)