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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Invest Dermatol. 2015 Jul 2;135(11):2834–2841. doi: 10.1038/jid.2015.255

Figure 2.

Figure 2

RPA and DDB2 chromatinization following treatment

a) 6-TG/UVA. HeLa, U2OS or U2OS-RPA21 cells grown for 48 h in 1 μM, 4 μM or 10 μM 6-TG, respectively to ensure equivalent DNA substitution were UVA irradiated (20 kJ/m2). Whole cell extracts were analysed by western blotting for RPA70.

b) Ciprofloxacin/UVA and Ofloxacin/UVA. U2OS or U2OS-RPA21 cells were treated with 0.5 mM ciprofloxacin or ofloxacin/UVA as indicated and irradiated with UVA (20 kJ/m2). Whole cell extracts were analysed as in A.

c) DDB2. U2OS cells treated with 6-TG for 24 h or fluoroquinolone for 1 h at the concentrations shown were UVA irradiated as indicated. Whole cell extracts prepared immediately after irradiation were analysed by western blotting for DDB2 or DDB1/ β-actin as loading controls.