Table 1.
Bacterial strains and plasmid used in this study
| Strain or plasmid | Relevant features | Source or reference |
|---|---|---|
| E. coli | ||
| DH5 | High-efficiency transformation | Bethesda Research Laboratories |
| SM10 | Mobilization of RK2 oriT plasmids | Simon et al. (1983) |
| B. pertussis | ||
| Tohama I | Patient isolate | Kasuga, et al. (1954) |
| BP536 | Tohama I, StrR, NalR | Stibitz and Yang (1991) |
| BP1526 | BP536, fha-lacZ, ptx-phoA, ΔbvgA-1215 | Chen, et al. (2010) |
| Plasmids | ||
| pSS3967 | luxCDABE promoter assay vector | Chen, et al. (2010) |
| pQC1023 | pSS3967:: Pfim2-10C | Chen, et al. (2010) |
| pQC1025 | pSS3967:: Pfim2-12C | Chen, et al. (2010) |
| pSS4159 | pSS3967:: Pfim3-13C | Chen, et al. (2010) |
| pQC1157 | pSS3967:: Pfim3-15C | Chen, et al. (2010) |
| pHn | pSS3967:: hybrids (Pfim2-12C/Pfim3-15C) | This study |
| pQC1440 | pSS3967:: Pfim3-15C-ds0 | This study |
| pQC1441 | pSS3967:: Pfim3-15C-ds5 | This study |
| pQC1442 | pSS3967:: Pfim3-15C-ds10 | This study |
| pQC1443 | pSS3967:: Pfim3-15C-ds15 | This study |
| pQC1444 | pSS3967:: Pfim3-15C-ds20 | This study |
| pQC1840 | pSS3967:: Pptx a | This study |
| pQC1841 | pSS3967:: Pptx-DRE | This study |
| pQC1843 | pSS3967:: Pfha b | This study |
| pQC1844 | pSS3967:: Pfha-DRE | This study |
| pQC1631 | pSS3967:: PgapA* | This study |
| pQC1761 | pSS3967:: PgapA*-DRE | This study |
a
Cloned ptx fragment contains nucleotides 3988042 to 3988253 of the B. pertussis Tohama I genome and extends, relative to the start of transcription, from −192 to +21.
b
Cloned fhaB fragment contains nucleotides 1968586 to 1968731 of the B. pertussis Tohama I genome and extends, relative to the start of transcription, from −124 to +21.