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. 2015 Aug 20;1(6):664–677. doi: 10.1016/j.jcmgh.2015.08.003

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Sleep disruption alters transcription factor occupancy at the Cyp7a1 gene promoter. Chromatin immunoprecipitation (ChIP) assay depicting (A) HNF4α and (B) Dbp occupancy at the Cyp7a1 gene promoter over 24 hours. Samples were pooled from 5–6 mice per time point; white bar, control; black bar, sleep disrupted. Data were analyzed by two-way analysis of variance followed by Holm-Sidak post hoc comparison where appropriate. *P < .05 within time point. The x-axis indicates Zeitgeber time (ZT).