Table 1.

Specific thermocycling conditions and resulting DNA fragments

Gene variant	Primer sequence	Thermocycling conditions	Restriction enzyme	Fragments
NOS2A and −954G/C	F3′-CATATGTATGGGAATACTGTATTTCAG-5′	94°C/30 s, 61°C/45 s, 72°C/75 s, and 72°C/10 min	BsaI	GG (437 and 136 bp)
	R5′-TCTGAACTAGTCACTTGAGG-3′			GC (573, 437, and 136 bp)
				CC (573 bp)
VEGF and +936C/T	F3′-AAGGAAGAGGAGACTCTGCGCAGAGC-5′	94°C/30 s, 64°C/1 min, 72°C/75 s, and 72°C/5 min	NlaIII	CC (208 bp)
	R5′-TAAATGTATGTATGTGGGTGGGTGTGTCTACAGG-3′			CT (208, 122, and 86 bp)
				TT (122 and 86 bp)

Abbreviations: NOS, nitric oxide synthase; F, forward; R, reverse; s, second; min, minute; VEGF, vascular endothelial growth factor.