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. 2015 Jun 17;1(5):503–515. doi: 10.1016/j.jcmgh.2015.06.008

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Substance P (SP) regulates microRNA (miR)-221-5p expression through nuclear factor-κB (NF-κB) and c-Jun N-terminal kinase (JNK) pathways. MiR-221-5p expression was assessed by real-time polymerase chain reaction (RT-PCR). (A) NCM460-NK-1R (neurokinin-1 receptor) cells were pretreated with the NF-κB inhibitor caffeic acid phenethyl ester (CAPE, 10 μM) 30 minutes before SP exposure for 6 hours. (B) MiR-221-5p levels in cells transfected with siRNA-p65 for 48 hours before SP exposure of 6 hours. (C) NCM460-NK-1R cells were pretreated with the JNK inhibitor SP600125 30 minutes before SP exposure for 6 hours. (D) MiR-221-5p levels in cells transfected with siRNA-c-Jun for 48 hours before SP exposure for 6 hours. *P < .05, data show mean ± standard deviation of triplicate.