Table 1. Primers used in conventional, inverse and real-time PCR reactions.

Primer	Primer sequence (5' – 3')	Size (bp)	Target
Primers for conventional PCR
Ges F Ges R	GCGTTTTGCAATGTGCTC CCAGTTTTCTCTCCAACAACC	519	Internal fragment of bla GES gene
Primers for inverse PCR
Gcu14 FSQ Gcu14 RSQ	AGCAATCAACACACAGGGG CTCGCGTAAATGCACCGCTT	130	gcu14 circular form
GES FSQ GES RSQ	CAAGTTATTACACAACTCAT AGTGCGTGAATGAAGCGCAT	110	bla GES-1 circular form
AACA4 FSQ AACA4 RSQ	GCCAGGCATTCGAGCGAACAC ATTTAGCCACTCACATAGAGC	188	aacA4 circular form
Primers and probes for real time PCR (TaqMan)
RpsL F RpsL R Probe	GCCTGCGCTGCAAAACT TTTCGGCGTGGTGGTGTAT TCGTGGCGTATGCACC	67	rpsL transcripts
Gcu14 F Gcu14 R Probe	CATGCGCTTCTTGGTTCGT ACGCCAGCTTGGATGCAA ATGCCACGAGACCTT	56	gcu14 transcripts
Ges F2 Ges R2 Probe	GTGCAGCTTAGCGACAATGG CACAGAGTCGCCAATTTTACGA AATTGCAGCAGGTCCGCC	99	bla GES-1 transcripts
AacA4 F AacA4 R Probe	CAAGCGTTTTAGCGCAAGAGT TCGGCTCTCCATTCAGCATTG CCGTCACTCCATACATTG	59	aacA4 transcripts
Ges F3 AacA4 R2 Probe	TCCTGAGCACGGACAAATAG TCATAGAGCATCGCAAGGTC TTCCGTCACACTGCGCCTCA	134	Polycistronic transcripts