Fig 5. STAT3-HDAC3 and HDAC4 are divergent pathways to mediate leptin-induced BMP-2 mRNA expression in human primary chondrocytes.

Cells were kept as controls or treated with leptin for 1, 4, 8, and 24 h. (A) The associations of STAT3 with HDAC3 and HDAC4 were determined by an immunoprecipitation assay and Western blot and (B) the STAT3 acetylation was determined by Western blot. Results are representative of three independent experiments with similar results.