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. 2015 Dec 4;10(12):e0143541. doi: 10.1371/journal.pone.0143541

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© 2015 Bongio, Lampe

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — PhoA = alkaline phosphatase with no signal sequence. The other lanes have Asaia sequences fused to ‘phoA. Tsr = TonB-dependent siderophore receptor. Aap = amino acid permease. Tdr = TonB-dependent receptor plug. Gdh = glucose dehydrogenase. Ybp = YVTN beta-propeller repeat protein. Csy = cellulose synthase. Amu = aminomutase. Ptp = peptide transport permease. The top ELISA used an anti-PhoA-HRP antibody to detect the presence of the alkaline phosphatase protein. The bottom plate assay used a PNPP substrate, which turns yellow when cleaved by active PhoA.