Fig 6. Disruption of P. berghei development by anti-Pbs21 scFv-Shiva1 immunotoxin and scorpine constructs.

Three-day old A. stephensi mosquitoes were fed paratransgenic strains of Asaia expressing either a fusion constuct combining the siderophore receptor protein and PhoA (= control), or a fusion construct combining the siderophore receptor protein, an effector protein, and PhoA. These mosquitoes were then fed on an infective mouse and the parasite was allowed to develop for 14 days. The mosquitoes were then dissected, and oocysts on the midgut were counted for each individual. Each dot on the chart represents a single midgut count. The median number of oocysts for each data set is marked with a horizontal line. Inhibition = inhibition of oocyst formation relative to the control; Mean = mean oocyst number per midgut; Median = median oocyst number per midgut; N = number of mosquitoes analyzed; Prevalence = percentage of mosquitoes carrying at least one oocyst; Range = range of oocyst numbers per midgut; Tbp = transmission-blocking potential: 100 − {(prevalence of mosquitoes fed with recombinant P. agglomerans)/[prevalence of control mosquitoes] × 100}. The scorpine construct produced a significant inhibition of 80.1% calculated by the median oocyst number.