|
Strains
|
Relevant Characteristics
|
Source
|
|
E. coli Top10 F' |
F´{lacIq, Tn10(TetR)} mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu) 7697 galU galK rpsL (StrR) endA1 nupG (from life technologies #C3030-03) |
[31] |
|
E. coli K-12 MG1655
|
F- lambda- ilvG- rfb-50 rph-1 |
[32] |
|
Asaia bogorensis
|
Isolate from orchid tree flower, Indonesia (ATCC BAA-21) |
[24] |
|
Asaia sp. SF2.1
|
Wild type strain isolated from Anopheles mosquitoes. |
[26] |
|
Plasmids
|
Relevant Characteristics
|
Source
|
| pBBR1MCS-2 |
KanR, pBBR origin, oriT, MCS, used for plasmid construction |
[33] |
| pNB20 |
pBBR1MCS-2 with the lacZα gene replaced by a new MCS. |
This study |
| pNB51 |
pNB20 w/ promoter region changed to PnptII
|
This study |
| pNB90 |
pNB51 w/ 'phoA insert used for genomic library construction |
This study |
| pNB91 |
Random genomic library of Asaia DNA-‘phoA fusions |
This study |
| pNB92 |
pNB51 w/ 'phoA insert and MCS for gene fusion construction |
This study |
| pNB93 |
pNB92 w/ YVTN beta-propeller repeat gene cloned |
This study |
| pNB95 |
pNB92 w/ siderophore receptor gene cloned. |
This study |
| pNB96 |
Siderophore receptor—Pro-EPIP–‘phoA effector construct |
This study |
| pNB97 |
Siderophore receptor—scorpine–‘phoA effector construct |
This study |
| pNB99 |
Siderophore receptor—PLA2 –‘phoA effector construct |
This study |
| pNB101 |
Siderophore receptor—prochitinase–‘phoA effector construct |
This study |
| pNB102 |
Siderophore receptor—anti-Pbs21 scFv-Shiva1 –‘phoA construct |
This study |
| pNB103 |
Siderophore receptor—Shiva1 –‘phoA effector construct |
This study |
