Table 2. Swine-specific primers used for real-time PCR.
Gene | Primer (5' → 3') | Product Length | Tm (°C) |
---|---|---|---|
Bacteriodes | F: AACGCTAGCTACAGGCTT | 276 | 54 |
R: CAAATGTGGGGGACCTTC | |||
Firmicutes | F: GGAGYATGTGGTTTAATTCGAAGCA | 126 | 59 |
R: AGCTGACGACAACCATGCAC | |||
Lactobacillus | F: TGGATCACCTCCTTTCTAAGGAAT | 340 | 55 |
R: TGTTCTCGGTTTCATTATGAAAAAATA | |||
Bifidobacteria | F: GCG TGC TTA ACA CAT GCA AGT C | 129 | 55 |
R: CAC CCG TTT CCA GGA GCT ATT | |||
Enterobacteria | F: CATTGACGTTACCCGCAGAAGAAGC | 190 | 58 |
R: CTCTACGAGACTCAAGCTTGC |