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. 2015 Dec 4;10(12):e0143716. doi: 10.1371/journal.pone.0143716

Fig 3. Knockdown Lin28 via siRNA increased chemotherapeutic sensitivity.

Fig 3

(A).MRNA level of Lin28 after Lin28 knockdown were detected by qRT-PCR at 48h,72h post-transfection in MKN45/Lin28 C2 and MKN28/Lin28 C3. Each data point represents the mean±SD of three independent experiments. (**p<0.01,*p<0.05).(B). Protein level of Lin28 in MKN45/Lin28 C2 and MKN28/Lin28 C3 were detected by western-blot 72h after Lin28-siRNA transfection. (C). Chemo-sensitivity of MKN45/Lin28 and MKN28/Lin28 to OXA, PTX and ADM by MTT assay after Lin28 expression were knockdown. (D) and (E).Apoptosis change among MKN45/Lin28/si-Lin28, MKN45/Lin28/si-control, MKN28/Lin28/si-Lin28, MKN28/Lin28/si-control at OXA 3.2ug/ml,PTX 1uM.After Lin28 was knock-down,the apoptosis of MKN45/Lin28 were decreased. Each data point represents the mean±SD of three independent experiments. (*p<0.05).