Skip to main content
. 2015 Dec 4;10(12):e0144295. doi: 10.1371/journal.pone.0144295

Fig 6. Inhibition of PKR suppresses cytokine production in LX-2 cells.

Fig 6

The PKR inhibitor C16 or a vehicle was added to LX-2 cells at 1 hour prior to LPS stimulation at the indicated concentrations. Then, the cells were treated with 100 ng/mL LPS for 24 hours in the presence or absence of the PKR inhibitor C16. (A) Effects of inhibition of PKR on expression of cytokine mRNA. Cellular RNA was collected, and the mRNA levels of IL-6, MCP-1 and IL-1β were examined by real-time RT-PCR. GAPDH was used for normalization. (B) Effects of inhibition of PKR on expression of cytokines at protein level. IL-6 and MCP-1 protein levels were determined by ELISA. The results are expressed as mean ± SD. Minimum three replicates were performed for each set of experiments to compile the data as presented. Black and white columns indicate presence and absence of the PKR inhibitor C16, respectively.