Fig 5. Cell viability in Huh7 cells: Huh7 cells were pre-treated with lovastatin for 24h before everolimus was added, and the combination of both drugs was incubated for 48h (A) or 120h (B).

The single drugs were incubated for the same time as in combination. The mean percentage of cell viability, relative to the untreated control, ± SEM (error bars) is shown. (A) Treatment with 20 μM lovastatin or with 10 nM everolimus separately significantly reduced Huh7 cell viability. Combination treatment with 5–20 μM lovastatin and 10 nM everolimus significantly added to the effect of each drug alone. (B) Treatment with 5–20 μM lovastatin or with 10 nM everolimus alone significantly decreased Huh7 cell viability. Combination treatment with 20 μM lovastatin and 10 nM everolimus was significantly more effective than each drug separately. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001 (compared to the control); @ P ≤ 0.05, @@ P ≤ 0.01, @@@ P ≤ 0.001 (combination compared to each drug separately).