Figure 1. Formation of PEG-Peptide mRNA Polyplexes.
UTR mRNA (1 µg) was combined with 0, 0.01, 0.05, 0.1, 0.3, 0.7, or 1 nmol of peptide 1 (panel A) or peptide 2 (panel B) and electrophoresed on 1% agarose gel then stained with ethidium bromide. mRNA migration was prevented at 0.1 nmol of Peptide 1 and 2, indicating both peptides bind ionically to mRNA to form polyplexes.