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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Gene Ther. 2015 Jun 30;22(12):993–999. doi: 10.1038/gt.2015.68

Figure 2. Metabolic Stability of mRNA Polyplexes.

Figure 2

UTR mRNA (2 µg) was complexed with 1.6 nmol of peptide 1 (panel A) or peptide 2 (panel B) to form mRNA polyplexes that were digested with 0, 3, 10, 30, 100, 300, 1000, or 3000 ng/ml of RNase A in 20 µL 5 mM HEPES buffer, pH 7.4 at 37°C for 10 min. mRNA polyplexes were digested with proteinase K to remove PEG-peptides. Following phenol:chloroform:isoamyl alcohol extraction, mRNA was electrophoresed on 1% agarose gel then stained with ethidium bromide. Both PEG-peptides were found to protect mRNA from RNase digestion up to 100 ng/mL, whereas naked mRNA was completely digested with 3 ng/ml.