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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Gene Ther. 2015 Jun 30;22(12):993–999. doi: 10.1038/gt.2015.68

Figure 5. Serum Incubation of mRNA Polyplexes.

Figure 5

UTR mRNA (1 µg) was combined with 0.8 nmol peptide 1 or 2 and incubated in mouse serum (20 µl) for 30 min. mRNA polyplexes were diluted with saline, dosed hydrodynamically in mice, and assayed for luciferase expression by bioluminescence imaging at 24 hours. Naked UTR mRNA and peptide 2 UTR mRNA polyplexes produced no expression, whereas peptide 1 polyplexes produced luciferase expression comparable to pGL3. Statistically significant differences relative to UTR mRNA peptide 1 polyplex dose are marked, with * p ≤ 0.05, **** p ≤ 0.0001.