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. Author manuscript; available in PMC: 2016 Dec 4.
Published in final edited form as: Glia. 2015 Jun 4;63(11):1982–1996. doi: 10.1002/glia.22868

Figure 7. Elevated chronic astrogliosis subsequent to acute Treg depletion.

Figure 7

(A) IHC staining of brain sections obtained from MCMV-infected Foxp3-DTR-GFP transgenic mice with (+DTx) or without (−DTx) DTx treatment for expression of GFAP at 40 dpi. (B) Brain homogenates were collected from uninfected or untreated and DTx-treated animals; and cDNA was synthesized from 1.0 µg of total RNA. Fold change in GFAP mRNA expression relative to uninfected animals was quantified using the 2−ΔΔCt method and normalized to the housekeeping gene HPRT. Data shown are representative of two experiments using three animals/time point/experiment. (C) Differences in GFAP protein levels in brains with or without infection and Treg depletion (±DTx) were quantified at 40 dpi using ELISA. Pooled data presented are derived from three to nine animals/group. *P ≤ 0.05 vs. untreated uninfected. ††P ≤ 0.01 vs. MCMV.