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. 2015 Dec 4;9(6):064112. doi: 10.1063/1.4936927

FIG. 1.

FIG. 1.

Schematic illustration of the experiment procedures and characterization of the microfluidic device. (a) The microfluidic device used in the current study, which was composed of four layers, a PDMS stencil, a PDMS micropillar substrate, a thin PDMS membrane (not shown), and a polystyrene culture dish. The PDMS stencil was sealed to the micropillar substrate, and each type of cells was seeded into the appropriate region (from left to right, HaCaT, ESF-1, and HUVEC cells, respectively). (b) The PDMS stencil was peeled off after the cells were attached and cultured on the micropillar substrate for 24 h. (c) A typical fluorescence image of well-distributed cells after the stencil was just peeled off. To visualize clearly the coexistence of cells, HaCaT (left), ESF-1 (middle), and HUVEC (right) cells were specifically stained using CellTracker Orange, CellTracker Green, and CellTracker Orange, respectively. (d) A typical fluorescence image of cells after wounding, corresponding to 36 h. (e) SEM image of the micropillar substrate. (f) Enlarged image of the square in the dotted lines in (e).