Fig. 3.

Agarose gel electrophoresis of PCR products from 8 different amplification protocol sharing the same primer pair (500 bp DNA ladder fragment), template (genomic DNA from Bacillus anthracis 34F2 Sterne strain) and annealing temperature range (58–68 °C). Conditions applied to well No 45 (marked by arrow) were subsequently selected for optimum amplification yield. Numbers indicate individual PCR reactions with wells characterized by M representing DNA 100 bp ladder (Product No. SM0323, Fermentas, USA).