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Analysis of large cerebral arteries. a. H&E of major branches of the circle of Willis and vertebral arteries (VA). Note marked splitting between the intima and media in the ACTA2 mutant (arrows), resulting in complete separation of the tunics in the right MCA (insert shows media). b-c. The thickness of intima (b) and of media (c) were measured at maximum and mean layer thickness, respectively. Control and mutant datasets are compared by two-way ANOVA
