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. 2015 Dec 4;8:82. doi: 10.1186/s13041-015-0174-2

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© Watanabe et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — IGFBP-3 expression in human brains. Brain homogenates of temporal cortexes from AD patients (AD, n = 5) and age-matched non-AD controls (control, n = 5) were analyzed via western blot using anti-IGFBP3 and β-actin antibodies (a). The band densities were quantified using Image J. The IGFBP3/β-actin ratio was calculated and analyzed using Mann-Whitney’s U test. The IGFBP3/β-actin ratio was significantly increased in the brains of AD patients (b, 188.1 ± 23.9 %, p < 0.05). The data represent the mean ± SE. * indicates statistically significant differences (*p < 0.05)