Table 2. Pronase E glycan map for alpha-2 macroglobulin§.
| Glycopep. Mass (Da) | RT | Glycosite | Peptide Sequence | Hex-HexNAc-Fuc-Neu5Ac | |
|---|---|---|---|---|---|
| Exp. | Cal. | ||||
| 3316.301 | 3316.312 | 37.3 | 55 | SYLNETVTVS | 5-4-0-2 |
| 2812.052 | 2812.043 | 25.8 | 55 | NETV | 5-4-1-2 |
| 3462.358 | 3462.370 | 37.6 | 55 | SYLNETVTVS | 5-4-1-2 |
| 2601.042 | 2601.055 | 23.2 | 70 | SVRGNR | 5-4-0-1 |
| 2706.063 | 2706.050 | 24.2 | 70 | RGNR | 5-4-0-2 |
| 2892.137 | 2892.150 | 24.7 | 70 | SVRGNR (VRGNRS) | 5-4-0-2 |
| 3134.26 | 3134.277 | 26.4 | 70 | LESVRGNR | 5-4-0-2 |
| 3308.343 | 3308.341 | 25.1 | 70 | SLESVRGNRS | 5-4-0-2 |
| 2747.102 | 2747.113 | 25.9 | 70 | SVRGNR (VRGNRS) | 5-4-1-1 |
| 2989.229 | 2989.239 | 26.9 | 70 | LESVRGNR | 5-4-1-1 |
| 3163.301 | 3163.303 | 24.0 | 70 | SLESVRGNRS | 5-4-1-1 |
| 2852.105 | 2852.108 | 27.0 | 70 | RGNR | 5-4-1-2 |
| 3038.19 | 3038.208 | 28.3 | 70 | SVRGNR (VRGNRS) | 5-4-1-2 |
| 2852.105 | 2852.096 | 26.8 | 70 | VRGN | 5-5-0-2 |
| 2458.896 | 2458.904 | 24.3 | 247 | EEEMNVS | 5-4-0-0 |
| 2749.99 | 2749.999 | 26.6 | 247 | EEEMNVS | 5-4-0-1 |
| 2855.007 | 2854.994 | 28.4 | 247 | EEEMN | 5-4-0-2 |
| 3041.084 | 3041.095 | 28.7 | 247 | EEEMNVS | 5-4-0-2 |
| 3803.496 | 3803.478 | 48.9 | 247 | ILEEEMNVSVCG (LEEEMNVSVCGL) | 6-5-1-1 |
| 2595.938 | 2595.943 | 21.4 | 396 | SNAT | 5-4-0-2 |
| 2922.051 | 2922.069 | 35.4 | 396 | YYSNAT | 5-4-0-2 |
| 3135.133 | 3135.144 | 25.0 | 396 | EANYYSNA (SNATTDEHG) | 5-4-0-2 |
| 3138.151 | 3138.144 | 50.2 | 396 | YYSNATTD | 5-4-0-2 |
| 2450.92 | 2450.905 | 19.3 | 396 | SNAT | 5-4-1-1 |
| 2579.887 | 2579.948 | 22.8 | 396 | NATTD | 5-4-1-1 |
| 2741.998 | 2742.001 | 25.2 | 396 | SNAT | 5-4-1-2 |
| 2870.976 | 2871.043 | 26.2 | 396 | NATTD | 5-4-1-2 |
| 2198.868 | 2198.857 | 18.8 | 410 | INTT | 4-4-0-1 |
| 2285.884 | 2285.889 | 20.2 | 410 | SINTT | 4-4-0-1 |
| 2360.911 | 2360.910 | 19.3 | 410 | INTT | 5-4-0-1 |
| 2447.952 | 2447.942 | 21.2 | 410 | SINTT | 5-4-0-1 |
| 2594.991 | 2595.010 | 27.5 | 410 | FSINTT | 5-4-0-1 |
| 2652.026 | 2652.005 | 23.9 | 410 | INTT | 5-4-0-2 |
| 2506.979 | 2506.968 | 23.6 | 410 | INTT | 5-4-1-1 |
| 4828.829 | 4828.841 | 49.9 | 410 | QFSINTTNVMGT | 7-6-0-4 |
| 1950.746 | 1950.731 | 18.4 | 869 | GNVN | 4-3-0-1 |
| 2153.816 | 2153.810 | 18.3 | 869 | GNVN | 4-4-0-1 |
| 2602.045 | 2602.043 | 29.6 | 869 | NVNFTVSA (SLGNVNFT) | 4-4-0-1 |
| 1618.616 | 1618.609 | 16.1 | 869 | GNVN | 5-2-0-0 |
| 2066.835 | 2066.841 | 27.4 | 869 | NVNFTVSA(SLGNVNFT) | 5-2-0-0 |
| 2112.789 | 2112.784 | 17.2 | 869 | GNVN | 5-3-0-1 |
| 2315.875 | 2315.863 | 19.1 | 869 | GNVN | 5-4-0-1 |
| 2515.972 | 2515.979 | 23.0 | 869 | SLGNVN | 5-4-0-1 |
| 2764.092 | 2764.096 | 29.6 | 869 | NVNFTVSA (SLGNVNFT) | 5-4-0-1 |
| 2483.902 | 2483.894 | 20.4 | 869 | NF | 5-4-0-2 |
| 2606.964 | 2606.959 | 23.4 | 869 | GNVN | 5-4-0-2 |
| 3055.179 | 3055.191 | 33.4 | 869 | NVNFTVSA (SLGNVNFT) | 5-4-0-2 |
| 2274.844 | 2274.837 | 18.5 | 869 | GNVN | 6-3-0-1 |
| 2723.063 | 2723.069 | 29.4 | 869 | NVNFTVSA (SLGNVNFT) | 6-3-0-1 |
| 2531.941 | 2531.938 | 22.6 | 991 | NETQQ | 5-4-0-1 |
| 2694.976 | 2694.975 | 23.4 | 991 | NETQ | 5-4-0-2 |
| 2823.053 | 2823.033 | 23.8 | 991 | NETQQ | 5-4-0-2 |
| 2936.129 | 2936.117 | 25.7 | 991 | LNETQQ (NETQQL) | 5-4-0-2 |
| 2958.068 | 2958.091 | 49.5 | 991 | DYLNET | 5-4-0-2 |
| 3170.236 | 3170.243 | 49.9 | 991 | VLDYLNET | 5-4-0-2 |
| 3895.589 | 3895.614 | 36.5 | 991 | DYLNETQQLTPEIK | 5-4-0-2 |
| 4107.748 | 4107.723 | 37.7 | 991 | LFAPNIYVLDYLN | 5-5-1-2 |
| 2704.068 | 2704.059 | 20.0 | 1424 | DKVSNQT | 5-4-0-1 |
| 2789.141 | 2789.148 | 19.8 | 1424 | KVSNQTLS | 5-4-0-1 |
| 2565.93 | 2565.932 | 21.4 | 1424 | NQT | 5-4-0-2 |
| 2995.151 | 2995.155 | 23.3 | 1424 | DKVSNQT | 5-4-0-2 |
| 3080.232 | 3080.244 | 22.4 | 1424 | KVSNQTLS | 5-4-0-2 |
| 2850.12 | 2850.117 | 23.4 | 1424 | DKVSNQT | 5-4-1-1 |
| 2935.203 | 2935.206 | 23.7 | 1424 | KVSNQTLS | 5-4-1-1 |
| 3141.242 | 3141.212 | 24.2 | 1424 | DKVSNQT | 5-4-1-2 |
| 2144.800 | 2144.799 | 17.5 | 247 (869) | NV | 5-4-0-1 |
| 2423.867 | 2423.858 | 19.4 | 396 (1424) | SN | 5-4-0-2 |
| 2520.929 | 2520.922 | 20.4 | 396 (410) | SNATTD (QFSIN) | 5-4-0-1 |
| 2812.052 | 2812.017 | 25.8 | 396 (410) | SNATTD (QFSIN) | 5-4-0-2 |
| 2666.993 | 2666.980 | 22.2 | 396 (410) | SNATTD (QFSIN) | 5-4-1-1 |
| 2958.079 | 2958.075 | 27.5 | 396 (410) | SNATTD (QFSIN) | 5-4-1-2 |
| 3881.579 | 3881.562 | 36.0 | 396 (410) | NATTDEHGLVQFSI (ATTDEHGLVQFSIN) | 5-4-1-2 |
| 2361.881 | 2361.869 | 17.5 | 410 (1424) | NTTN (SNQT) | 5-4-0-1 |
| 2474.958 | 2474.953 | 20.1 | 410 (1424) | INTTN (DKVSN, SNQTL) | 5-4-0-1 |
| 2652.977 | 2652.964 | 21.7 | 410 (1424) | NTTN (SNQT) | 5-4-0-2 |
| 2507.939 | 2507.927 | 19.6 | 410 (1424) | NTTN (SNQT) | 5-4-1-1 |
| 2275.839 | 2275.821 | 18.3 | 55 (991, 247) | NET (NET, MNV) | 5-4-0-1 |
| 2566.912 | 2566.916 | 22.5 | 55 (991, 247) | NET (NET, MNV) | 5-4-0-2 |
| 2421.888 | 2421.879 | 20.1 | 55 (991, 247) | NET (NET, MNV) | 5-4-1-1 |
| 2712.978 | 2712.974 | 26.8 | 55 (991, 247) | NET (NET, MNV) | 5-4-1-2 |
| 2549.952 | 2549.949 | 20.7 | 70 (869) | RGN (NVN) | 5-4-0-2 |
| 2695.978 | 2695.995 | 25.4 | 70 (869) | RGN (NVN) | 5-4-1-2 |
| 3426.349 | 3426.371 | 49.3 | 70(991) | GNRSLFTDLEA(VLDYLNETQQ) | 5-4-0-2 |
| 2404.899 | 2404.900 | 18.4 | 869 (70) | NVN (RGN; GNR) | 5-4-1-1 |
§
No glycoform abundance is listed because the same glycoform is spread out on different peptide sequences