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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Cell Metab. 2015 Oct 17;22(6):1045–1058. doi: 10.1016/j.cmet.2015.09.013

Figure 6.

Figure 6

Leptin stimulation of MCs and pro- and anti-inflammatory signaling and cytokine expression. A. ELISA determined culture medium histamine levels in WT and ob/ob BMMCs treated with or without leptin or IgE. ELISA determined medium histamine (B) and IFN-γ and IL4 levels (C) in WT and ob/ob BMMCs treated with and without leptin, anti-leptin antibody-treated leptin or control IgG1-treated leptin as indicated. D. Immunoblot of pro-inflammatory (left) and anti-inflammatory (right) signaling molecules in BMMCs from WT and ob/ob mice treated with or without leptin. E. Immunoblot of pro-inflammatory signaling molecules in BMMCs from WT and ob/ob mice treated with or without IgE. ELISA determined culture medium histamine (F) and IFN-γ and IL4 (G) in WT and ob/ob BMMCs treated with or without leptin, different signaling molecule inhibitors, or vehicle DMSO.