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. Author manuscript; available in PMC: 2017 Jan 10.
Published in final edited form as: J Proteomics. 2015 Oct 19;131:122–130. doi: 10.1016/j.jprot.2015.10.019

Figure 1.

Figure 1

The strategies implemented ad hoc to maximize the coverage of the proteome consist of a subcellular fractionation based on increasing centrifugation speeds followed by exhaustive protein-level separation on SDS-PAGE; and OFFGEL peptide-level separation from a pool of peptides from different growing and culture conditions. The MS output results, combined with additional MS datasets from works on the secreted and surface proteomes, along with the datasets in the first atlas were all processed with the TPP.