Table 2.
Sequences of primers used in qPCR.
| Gene | Primer | Sequence 5′-3′ | Amplicon length | Accession number | Annealing temperature |
|---|---|---|---|---|---|
| GAPDH | F: | GATGCCCCAATGTTTGTGA | 250 | NM_001163856.1 | 61,7°C |
| R: | AAGCAGGGATGATGTTCTGG | ||||
|
| |||||
| p53 | F: | TACTCCCCTGCCCTCAACAA | 252 | U37120.1 | 57,6°C |
| R: | AGGAATCAGGGCCTTGAGGA | ||||
|
| |||||
| p21 | F: | GAAGAGAAACCCCCAGCTCC | 241 | XM_003365840.2 | 52,1°C |
| R: | TGACTGCATCAAACCCCACA | ||||
|
| |||||
| BAX | F: | TTCCGACGGCAACTTCAACT | 150 | XM_005607505.1 | 60,5°C |
| R: | GGTGACCCAAAGTCGGAGAG | ||||
|
| |||||
| CAS-9 | F: | TCCTACTCCACCTTCCCAGG | 150 | XM_005607505.1 | 60,8°C |
| R: | CTCCGAAACAGCGTGAGCTA | ||||
|
| |||||
| BCL-2 | F: | TTCTTTGAGTTCGGTGGGGT | 164 | XM_001490436.2 | 65,0°C |
| R: | GGGCCGTACAGTTCCACAA | ||||
|
| |||||
| BMP-2 | F: | CGTCCTGAGCGAGTTCGAGT | 249 | XM_001493895.4 | 60,0°C |
| R: | CGCCGGGTTGTTTTTCCACT | ||||
|
| |||||
| RUNX-2 | F: | CCAAGTGGCAAGGTTCAACG | 165 | XM_005603968.1 | 60,2°C |
| R: | TGTCTGTGCCTTCTGGGTTC | ||||
|
| |||||
| COLL-1 | F: | GAAACTATCAATGGTGGTACCAAGT | 265 | XM_008524258.1 | 55,0°C |
| R: | AGCAGCCATCTACAAGAACAGT | ||||
|
| |||||
| COMP | F: | AGTGTCGCAAGGATAACTGCGTGA | 238 | NM_001081856.1 | 62,6°C |
| R: | TCCTGATCTGTGTCCTTCTGGTCA | ||||
|
| |||||
| COLL-2 | F: | ATTCCTGGAGCCAAAGGATCTGCT | 148 | XM_005611082.1 | 62,7°C |
| R: | TGAAGCCAGCAATACCAGGTTCAC | ||||
|
| |||||
| VIM | F: | GCAGGATTTCTCTGCCTCTT | 203 | NM_001243145.1 | 55,0°C |
| R: | TATTGCTGCACCAAGTGTGT | ||||
Sequences, amplicon length, and annealing temperature of the primer sets. GAPDH: glyceraldehyde 3-phosphate dehydrogenase; p53: tumor suppressor p53; p21: cyclin-dependent kinase inhibitor 1A; BAX: BCL-2-associated X protein; BCL-2: B-cell lymphoma 2; CAS-9: caspase-9; BMP-2: bone morphogenetic protein 2; RUNX-2: runt-related transcription factor 2; COLL-1: collagen type I; COMP: cartilage oligomeric matrix protein; COLL-2: collagen type II; VIM: vimentin.