Figure 6. JNK interactome based on the presence of JIP1‐type motifs.

1. Low‐level functional classification of JIP1‐type motif‐bearing proteins. This analysis reveals that cytoskeletal regulation is at least as important aspect of JNK1 signaling as control of gene expression. The best 100 hits on the JIP1‐type motif list contain many proteins whose docking motif is already known (blue letters), or was validated in our dot‐blot essays (red letters). Only categories that contain more than a single protein are shown.
2. High‐level functional classification of the best‐ranking 100 JIP1‐type motif. The analysis reveals several major functions associated with JNK kinases. The role of JNK in inflammation, neuronal development, metabolic regulation, or apoptosis is already known from cell‐based experiments. Motif search results, in addition to providing the mechanistic basis for these regulatory processes, also suggest novel functions. The full functional clustering of JIP1‐type D‐motifs along with NFAT4‐ and MEF2A‐type motifs is shown in Table EV2.