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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: J Immunol. 2015 Nov 13;195(12):5572–5581. doi: 10.4049/jimmunol.1501604

Fig. 2. CD8 memory phenotype cells are increased in B10.TCRδ−/− mice.

Fig. 2

Spleen cells from five week-old female mice were analyzed by flow cytometry. Each column shows the mean value obtained from multiple mice; errors bars indicate SEM. A. After gating on CD3+ TCRβ+ cells, the proportion expressing CD4 or CD8 was determined, using 4 mice per group. B. Using the proportions obtained in A, the number of CD4+ and CD8+ spleen cells in each mouse was calculated; the average obtained from 4 individual mice is shown. C. After gating on CD3+ TCRβ+ CD4+ spleen cells (left), or CD3+ TCRβ+ CD8+ spleen cells (right), the proportion having the T effector memory phenotype (TEM) was determined as those cells staining brightly with anti-CD44 and dimly with anti-CD62L. The proportion having the T central memory cell phenotype was determined as those cells staining brightly for both CD44 and CD62L. For each group, data were averaged from 3–6 five week-old mice. D. As for C, except that 6–10 females aged 18–30 weeks were used to calculate averages. E. Representative flow cytometry plots of samples analyzed in A and B, including the gating strategy used to test for memory phenotype αβ T cells.