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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: J Immunol. 2015 Nov 13;195(12):5572–5581. doi: 10.4049/jimmunol.1501604

Fig. 3. In B10.TCRδ−/− mice, the frequency of CD8+ T cells producing inflammatory cytokines is elevated.

Fig. 3

Spleen cells were stimulated with PMA/ionomycin for 4 hours at 37°C in the presence of Brefeldin A. After surface staining with anti-CD3, anti-TCR-β, anti-CD8, and anti-CD4 mAbs, cells were fixed and permeabilized, and then stained with the indicated anti-cytokine mAb. The percent of CD3+ TCR-β+ CD8+ or CD3+ TCR-β+ CD4+ cells staining with each anti-cytokine mAb was determined by flow cytometric analysis. Means obtained from 4 five week-old female mice per group are shown; error bars indicate SEM.