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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Cancer Prev Res (Phila). 2015 Oct 20;8(12):1174–1183. doi: 10.1158/1940-6207.CAPR-14-0334

Figure 5. Locally-produced leptin and adiponectin modulate mammary stem cell self-renewal in vitro.

Figure 5

A. Primary mammospheres from 5 subjects were grown in the presence of recombinant leptin (100 ng/mL) or adiponectin (25 μg/mL) and counted. Spheres were dissociated and re-plated to form secondary mammospheres. The percent change from baseline is a measure of the rate of stem cell self-renewal. Leptin is sufficient to increase the rate of mammary stem cell self-renewal by 7–25%, whereas adiponectin inhibits self-renewal and decreases the stem cell population. N=4 replicates ± STD. B. Pooled primary mammospheres representing 12 random samples were grown in the presence of adipose conditioned media from 15 human specimens. Spheres were dissociated and re-plated to form secondary mammospheres. There was a strong linear relationship between the leptin/adiponectin ratio in adipose-conditioned media and human mammary stem cell self-renewal. Cultures were grown in quadruplicate, and the mean % change over negative control is shown.