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. Author manuscript; available in PMC: 2015 Dec 6.
Published in final edited form as: J Biomater Appl. 2015 Jul 15;30(5):558–567. doi: 10.1177/0885328215594704

Figure 2.

Figure 2

Investigation of the effect of orientation of anti-mAb on the binding of the immune complex of anti-BMP2/BMP2 to target cells. Flow cytometric analysis of binding of the immune complex between anti-BMP-2 mAb/recombinant protein-G/BMP-2, BMP-4 or BMP-7/BMP-2 cellular receptor on C2C12 cells. Fluorochrome-labeled cells were analyzed by flow cytometer and the mean fluorescence intensity (MFI) of PE was calculated. Controls included cells alone (−) or substitution of chimeric anti-BMP2 mAb with isotype-matched Ab (Iso mAb). The MFI of flowcytometric analysis showed significant binding between PG/chimeric antibody complex and BMP2.

**p < 0.01, and ***p < 0.001.