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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: J Immunol. 2015 Nov 13;195(12):5780–5786. doi: 10.4049/jimmunol.1501023

FIGURE 5.

FIGURE 5

mTOR, but not p70S6K1 or p70S6K2, is involved in the degradation of Mfn2 protein. (A) HEK293A cells were co-transfected either empty vector (pUSE) or CA-Akt plasmids together with flag-tagged Mfn2 plasmid, with or without pretreatment with 20ng/ml and 100ng/ml of rapamycin. (B) HEK293A cells were co-transfected with empty vector (pCDNA3), WT-mTOR plasmid, or KD-mTOR plasmids together with flag-tagged Mfn2 plasmid. (C) HEK293A cells were co-transfected with either empty vector (pCDNA3), WT-mTOR plasmids together with flag-tagged Mfn2 plasmid, with or without pretreatment of 20ng/ml and 100ng/ml of rapamycin. (D) HEK293A cells were co-transfected with either empty vector plasmids (pUSE, PRK7, or pcDNA3), WT-S6K1 plasmid or WT-S6K2 plasmid together with Flag-tagged Mfn2 plasmid. After 24 hours, whole cell lysates were prepared and 25 µg of protein from the whole cell lysates were analyzed by western blot analysis in (A), (B), (C) and (D). One representative experiment out of two experiments for each panel is shown here.