FIGURE 2.

TA-DC FOXO3 forms a protein complex with NF-κB RelA. (A) FOXO3 or (B) NF-κB RelA was immunoprecipitated from WT DC or TA-DC lysates. The immunoprecipitated proteins were then analyzed by Western blot for FOXO3 and NF-κB family member proteins. (C) Fluorescence anisotropy indicates that RelA interacts directly with FOXO3. (D) NF-κB RelA responses were decreased in DC2.4 cells transfected to overexpress FOXO3 as measured by RelA luciferase reporters. Results are displayed as one independent experiment and are representative of three trials. **p < 0.01, ****p < 0.001.