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Colocalization diminishes when less protein is present. HEK 293T cells were transfected with 1.5 mg FOXO3 DNA and decreasing concentrations of RelA and analyzed (A) by Amnis Image Stream (original magnification ×40) for visual confirmation and (B) i.p. Western blot for quantitative analysis of cells containing colocalization. Results are representative of at least three individual experiments.
