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. Author manuscript; available in PMC: 2016 Dec 17.
Published in final edited form as: Neuroscience. 2015 Oct 19;311:166–179. doi: 10.1016/j.neuroscience.2015.10.028

Fig. 2. Pain Related Behavioral Assessments.

Fig. 2

A. Footpad mechanical hypersensitivity. The 50% paw withdrawal threshold (g) to mechanical stimulation (von Frey fiber test) was similar between rats fed standard chow and the AHF fed group at baseline. In the AHF group, the mechanical threshold was progressively decreased beginning in week 3 and reached maximum hypersensitivity in week 5. The paw mechanical allodynia was maintained through the entire experimental time course (**** p<0.0001, two-way ANOVA, Bonferroni post-test).

B. Abdominal heat hypersensitivity. The abdominal withdrawal latency (ABWL) was similar for standard chow control and AHF fed groups at baseline through week 1. Secondary heat hyperalgesia developed on the abdominal skin by the second week on the AHF diet. The abdominal withdrawal latency (ABWL) of the AHF induced pancreatitis group decreased from a baseline of 13.70 ± 1.1 s to 6.58 ± 0.32 s. This hypersensitivity level was maintained throughout the remainder of the experiment. This difference was statistically significant compared to the control chow fed group (****p<0.0001, two-way ANOVA, Bonferroni post-test).