Figure 2.

Frequency and Gene Expression Signature of Vaccine-Induced βgal-Specific CD8⁺ T Cells

C57BL/6 mice were immunized i.v. with 1 × 10⁹ pfus Ad-LacZ.

(A) Representative flow cytometry plots of tetramer staining for βgal₉₆- and βgal₄₉₇-specific CD8⁺ T cells on 21 and 100 days postimmunization in the spleen. Mean percentage of live tetramer-positive CD8⁺ T cells is indicated (n = 10; mean ± SEM).

(B) Time course for βgal₉₆- (red) and βgal₄₉₇- (blue) specific CD8⁺ T cells. Splenocytes from 0, 21, 50, and 100 days postimmunization were stained with tetramers and analyzed by flow cytometry. Mean percentages of live tetramer-positive CD8⁺ T cells are indicated (n = 5; mean ± SEM).

(C) Venn diagram showing the number of differentially expressed genes between βgal₉₆-specific CD8⁺ T cells 21 (yellow) and 100 (red) days postimmunization and βgal₄₉₇-specific CD8⁺ T cells 21 (orange) and 100 (blue) days postimmunization. Filter criteria of at least 2-fold change with p ≤ 0.05 compared to naive CD8⁺ T cells are shown. Upregulated genes are indicated in red and downregulated genes in blue.

See also Figure S2.