Figure 4.

Distinct Cytokine and Chemokine Receptor Expression and Individual Transcription Regulator Profile on Expanded CD8⁺ T Cells Compared to Conventional Memory CD8⁺ T Cells

(A) Heatmap showing mRNA expression levels of cytokine and chemokine receptors measured by microarray analysis of naive, M38-, and M45-specific CD8⁺ T cells days 7 and 50 postinfection (left panel) and naive, βgal₉₆-, and βgal₄₉₇-specific CD8⁺ T cells days 21 and 100 postvaccination (right panel). Shades of red indicate upregulated genes, and shades of blue indicate downregulated genes. Filter criteria of at least 2-fold changes with p ≤ 0.05, compared to naive CD8⁺ T cells, are shown.

(B) Longitudinal flow cytometry analysis showing the percentage of IL-6Rα (CD126), CX3CR1, and CXCR3 expression on M38/βgal₉₆- (red) and M45/βgal₄₉₇-specific CD8⁺ T cells (blue) in the spleen at days 0, 7, 21, 50, and 100 postinfection/immunization (n = 6–8; ±SEM). Histograms show expression of indicated markers, gated on live naive (green) M38/βgal₉₆- (red) and M45/βgal₄₉₇-specific CD8⁺ T cells (blue).

(C) Heatmap showing mRNA expression levels of transcriptional regulators measured by microarray analysis of naive, M38-, and M45-specific CD8⁺ T cells days 7 and 50 postinfection (left panel) and naive CD8⁺ T cells and βgal₉₆- and βgal₄₉₇-specific CD8⁺ T cells days 21 and 100 postvaccination (right panel). Shades of red indicate upregulated genes, and shades of blue indicate downregulated genes. Filter criteria of at least 2-fold changes with p ≤ 0.05, compared to naive CD8⁺ T cells, are shown.

(D) Flow cytometry analysis showing the MFI of cells expressing the TFs EOMES and Tbet on M38- (red) and M45-specific CD8⁺ T cells (blue; n = 6–8; ±SEM) and on βgal₉₆- (red) and βgal₄₉₇-specific (blue) CD8⁺ T cells in the spleen (n = 5–9; ±SEM). Histograms show expression of EOMES and Tbet and are gated on live naive (green), M38/βgal₉₆-specific CD8⁺ T cells (red), and M45/βgal₄₉₇-specific CD8⁺ T cells (blue).

See also Figures S3 and S4.