Figure 5.

Effects of 30 nmol/L MTH treatment on erythroid differentiation markers. K562 (white boxes) and clone 12 (black boxes) cells were treated in the absence or presence of 30 nmol/L MTH. (A) Effects on the increase in the proportion of benzidine-positive cells (%) evaluated after 4 and 5 days of cell culture. (B, C) After RNA extraction, expression of the following mRNAs was analyzed in 4-day MTH-treated cells: γ-globin, trfR, DNMT (EBP49), NFE2L3 (B); and SCL4A1 (band-3), ALAS-2, GYPA (C). Data represent averages ± SD of four independent experiments.