Supplementary Table E1.
TaqMan assays obtained from Applied Biosystems and PrimeTime qPCR Assays obtained from Integrated DNA Technologies employed in RT-qPCR experiments (primer and probe sets)
| Gene name | Species | Company | Assay ID |
|---|---|---|---|
| B-Cell lymphoma/leukemia 11A (BCL11A)a | Human | Applied Biosystemsb | Hs00256254_m1 |
| BCL11Ac | Human | Applied Biosystems | Hs00250581_s1 |
| ζ-Globin | Human | Applied Biosystems | Hs00923579_m1 |
| Nuclear factor erythroid 2-like 3 (NFE2L3) | Human | Applied Biosystems | Hs00852569_g1 |
| δ-Aminolevulinate synthase 2 (ALAS-2) | Human | Applied Biosystems | Hs00163601_m1 |
| Dematin actin binding protein (DNMT [EBP49]) | Human | Applied Biosystems | Hs00157387_m1 |
| Solute carrier family 4 member 1 (SCL4A1 [band3]) | Human | Applied Biosystems | Hs00978603_m1 |
| 18S ribosomal RNA | Human | Applied Biosystems | 4310893E |
| Ribosomal protein L13a (RPL13A) | Human | Applied Biosystems | Hs03043885_g1 |
| Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) | Mouse | IDTd | Mm.PT.39a.1 |
a
This assay was used for amplification of BCL11A transcripts in K562(BCL11A-XL) clones.
b
Applied Biosystems, Monza, Italy.
c
This assay was used for amplification of BCL11A transcripts in erythroid precursor cells from β-thalassemia patients.
d
Integrated DNA Technologies, Coralville, IA.