Figure 3.

Brain edema and infarct volume at 48 h post ischemia are reduced by caspase inhibition. (a) Coronal brain slices showing the representative infarct area in a sample from control, caspase-6 inhibitor (Z-VEID)-, or caspase-8 inhibitor (Z-IETD)-treated brains. Brains were processed by TTC staining to detect the infarcted brain region (white area) within the remaining metabolically active brain tissue (red); (b) effect of control, caspase-6 inhibitor, or caspase-8 inhibitor treatment on infarct volume (percentage of unlesioned hemisphere); (c) effect of caspase inhibition on brain edema percentage relative to the uninjured (contralateral) hemisphere; (d) effect of caspase inhibition on mean brain water content of ipsilateral (injured) or contralateral (intact) cerebral hemispheres. Data in b, c, and d are presented as mean±S.E.M. *P<0.05 between control and experimental group; n=7 each group