Figure 2.

SOX4 overexpression promotes tumor growth in vivo. RT-PCR (a), qRT-PCR (b) and western blot (c) analysis of SOX4 protein in wild-type CaSki, mock-transfected CaSki (CaSki/Mock) and SOX4-overexpressed CaSki (CaSki/SOX4) cells. GAPDH served as loading controls. (d) In vivo analysis of the effect of SOX4 overexpression on CaSki tumor growth (n=5 in each group). (e) Tumor photographs from mice 35 days post implanted with CaSki/SOX4 and CaSki/Mock cells. (f) Weights of tumors from mice 35 days post implanted with CaSki/SOX4 and CaSki/Mock cells. (g) Immunohistochemistry analysis of SOX4 and Ki67 in tumors from mice 35 days post implanted with CaSki/SOX4 and CaSki/Mock cells (× 400). The data were shown as the mean±S.E.M. *P<0.05 compared with the control groups