Figure 5.

DNA-damaging agents modulate the expression of heat-shock proteins (Hsps) and mitochondrial energy proteins. HCT116 WT cells treated with doxorubicin (Dox; 10 μM) for 12 h were harvested in TriZol and total RNA was isolated. 2 μg of total RNA was used to convert to cDNA. Control and treated cDNAs were applied to heat-shock protein array (a) and mitochondrial energy metabolism array (b) and run on Applied Biosystems 7300 platform. Fold change relative to untreated control is presented. An average of five housekeeping genes were used to normalize the expression. (c and d) Equal amounts of total RNA were subjected to RNA-Seq library preparation followed by sequence reads (80 million). Data are presented as total transcript levels for heat-shock response (c) or OXPHOS (d) genes compared with control housekeeping genes