Figure 3.

Requirement of p53 for suppressing CD133 expression. (a) Western blot and qPCR analyses were performed using lysates of NCCIT cells that had been transfected with the indicated amounts of HA-p53-expressing or empty vector. (b) Western blot and qPCR analyses were performed using lysates of LoVo cells transfected with HA-p53-expressing vector for the indicated time. (c) Western blot and qPCR analyses were performed for various cancer cell lines that had been transfected with either CD133 siRNA or scramble siRNA-expressing vector. (d) mRNA and protein expression levels of H1299 cells treated with the indicated dose of Dox (left panel), after UV irradiation (40 J/M²; middle panel), or treatment with Nutlin-3 (5 μM; right panel). (e) Western blot and qPCR analyses were performed using lysates of H1299 cells that had been transfected with the indicated amounts of HA-p53-expressing or empty vector. The error bars represent the mean±S.D. n=3. *P<0.05, **P<0.01, and ***P<0.001; NS, not significant