Figure 2.

Wnt3a overexpression favors alternative lineages development. (a) Total fetal liver cells were co-cultured with OP9-WT/DL1 : 1 (top row) or with OP9-Wnt3a/DL11 : 1 (bottom row). Cells were harvested after 14 days of co-culture and were analyzed by FACS for B cells (B220 and CD19) and myeloid cells (CD11b and Gr1). The plots are pre-gated on CD45+ cells. Representative plots of two independent experiments are shown. (b) Collective data of all experiments are depicted. OP9-WT/DL (Ctrl) are depicted in white bars and OP9-Wn3a/Dl (Wnt3a) in black bars. The left bar graphs present percentage of B cells (B220+ CD19+) within the CD45+ gate, and right bar graphs present percentage of myeloid cells (CD11b+ Gr1+) within the CD45+ gate. (c) Absolute number of total CD45+ life cells after 14 days of co-culture are depicted. Data are mean±S.D. of six controls and five Wnt3a samples from two independent experiments. *P<0.05; **P<0.01; ***P<0.001