Figure 4.

Smad4-deficient T cells show aberrant CD8⁺ T-cell differentiation. (a) The expression of CD127 and KLRG1 in K^b-ova⁺CD8⁺ splenic T cells of Smad4^co/co;Lck-Cre mice and their littermate controls on day 7 of LM-OVA primary infection (n=6 per group). (b) The expression of CD127 and KLRG1 in K^b-ova⁺CD8⁺ splenic T cells of Smad4^co/co;Lck-Cre mice and their littermate controls 5 days after the secondary infection (n=6 per group). (c) Bone marrow chimeric mice were prepared and treated as described in Figure 2d. The expression of CD45.1 and CD127 or KLRG1 in K^b-ova⁺CD8⁺CD45.2⁺ splenic T cells at day 7 post infection was analyzed by flow cytometry (n=3). (d) Bone marrow chimeric mice were rechallenged with 1 × 10⁵ c.f.u. of LM-OVA 35 days after primary infection. The expression of CD45.1 and CD127 or KLRG1 in K^b-ova⁺CD8⁺CD45.2⁺ splenic T cells 5 days after the secondary infection was analyzed by flow cytometry (n=3). (e) The expression of CD62L and CD27 in K^b-ova⁺CD8⁺ splenic T cells of Smad4^co/co;Lck-Cre mice and their littermate controls on day 7 of LM-OVA primary infection (n=6 per group). (f) The expression of CD62L and CD27 in K^b-ova⁺CD8⁺ splenic T cells of Smad4^co/co;Lck-Cre mice and their littermate controls 5 days after the secondary infection. Data shown in this figure are representative of at least three independent experiments